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1.
Braz. j. biol ; 80(1): 12-22, Feb. 2020. graf
Article in English | LILACS | ID: biblio-1089299

ABSTRACT

Abstract Medicinal plants, such as E. pyramidale, are alternatives against infections and assist in the healing process of wounds. We evaluated the effects of Hydrogel of E. pyramidale on the healing of cutaneous wounds in animal models by morphological, morphometric and histological analyses, and elucidated major components. The ethanolic extract of E. pyramidale shoots was submitted to phytochemical analysis. For cicatrizing activity assay, Wistar rats were used, and the groups evaluated were hydrogel containing ethanolic extract of E. pyramidale at 2% compared to control groups. The treatment periods were 3, 7 and 21 days, and macroscopic and microscopic analyses were performed. Data were submitted to Analysis of Variance (p <0.05). Phytochemistry and quantification evidenced that flavonoids are main constituents and that they were evidenced by thin layer chromatography (TLC), high efficiency (HPLC) and infrared (IR) methods. In TLC, using flavonoid patterns, we observed pinocembrin. The hydrogel containing ethanolic extract of E. pyramidale at 2% was effective in wound regression. E. pyramidale can be used for the treatment of second intention wounds, and the effective healing may be due to a high flavonoid content.


Resumo As plantas medicinais, como E. pyramidale, são uma alternativa para combater infecções e ajudar o processo de cicatrização de feridas. Avaliar os efeitos do hidrogel de E. pyramidale na cicatrização de feridas cutâneas em modelos animais através de análises morfológicas, morfométricas e histológicas, bem como para elucidar os principais componentes. O extrato etanólico das partes aéreas de E. pyramidale foi submetido a análise fitoquímica. Para o ensaio de atividade cicatrizante, foram utilizados ratos Wistar e os grupos avaliados foram o hidrogel com extrato etanólico de E. pyramidale 2% em comparação com os grupos controle. Os períodos de tratamento foram 3, 7 e 21 dias e as análises macroscópicas e microscópicas foram realizadas e os dados submetidos à Análise de Variância (p <0,05). A fitoquímica e a quantificação indicaram que os flavonóides são os constituintes principais e que mesmos foram evidenciados na cromatografia de camada delgada (CCD), de alta eficiência (HPLC) e por infravermelho (IV). Em CCD, empregando padrões de flavonoides, foi observado a pinocembrina. O hidrogel com extrato etanólico de E. pyramidale 2% foi eficaz na regressão da ferida. E. pyramidale pode ser usado no tratamento de feridas de segunda intenção e a cura efetiva pode ser devido ao alto teor de flavonoides.


Subject(s)
Animals , Rats , Equisetum , Skin , Plant Extracts , Rats, Wistar , Hydrogels
2.
Arq. bras. med. vet. zootec. (Online) ; 70(1): 315-320, Jan.-Feb. 2018. tab, ilus
Article in Portuguese | LILACS, VETINDEX | ID: biblio-1038582

ABSTRACT

The octopus Octopus cf. vulgaris is a potential species to diversify aquaculture. Due to absence of balanced commercial diet, growth of the O. cf. vulgaris is based on natural diet with local and low-cost inputs. In Brazil, studies on experimental octopus ongrowing are recent and there is little available data. We evaluated the performance, survival and food consumption of O. vulgaris fed on mussel Perna perna for 20 days. Six octopuses with initial weight of 415±12.73g (mean±standard deviation) were divided into two groups (n=3 octopuses/group) according to the diet: MC Group (frozen mussels) and MV Group (live mussels). The Weight Gain of octopuses was 273.33±94.52g and 340.00±26.46g; the Absolute Growth Rate was 13.67±4.73 and 17.00±1.32g.dia-1 and the Specific Growth Rate of 2.95±0.58 and 2.64±0.37%.day-1 to MC and MV groups, respectively. There was no significant difference in performance between groups and the survival rate was 100%. Octopuses well accepted both diets and despite the amount of frozen mussels (129±31) was higher than in live mussels (100±19), there was no significant difference regarding the consumption between groups. Our results demonstrate that the mussel Perna perna can be used frozen or live as monodiet in O. cf. vulgaris ongrowing.(AU)


Subject(s)
Animals , Diet/veterinary , Perna , Octopodiformes/growth & development , Aquaculture/methods
3.
Arq. bras. med. vet. zootec. (Online) ; 70(2): 628-632, mar.-abr. 2018. ilus
Article in Portuguese | LILACS, VETINDEX | ID: biblio-910973

ABSTRACT

The inexistence of nutritionally adequate diets in paralarval rearing is the main bottleneck for commercial production of the common octopus Octopus cf. vulgaris. We report the feeding behavior of O. vulgaris Type II paralarvae fed on Artemia sp (0.1 individual. mL-1) nauplii enriched with microalgae Isocrysis galbana and Pavlova lutheri microalgae from 0 to 7 Day After Hatching (DAH).; metanauplii enriched with microalgae and DHA SELCO® lipid emulsion from the 8 DAH. The paralarvae showed active swimming and predation by the 14 DAH, feeding in the most superficial portion of the water column. From the 15 DAH, the paralarvae remained near the bottom and there a decrease in the consumption of artemia was observed. The mortality observed from the 18 DAH and mass mortality of paralarvae on 20 DAH can be attributed mainly to the nutritional composition of the diet. Studies analyzing the biochemical composition and ontogeny of the digestive system during the early life stages should shed some light on the running for an appropriate feeding protocol to the paralarval rearing.(AU)


Subject(s)
Animals , Feeding Behavior , Octopodiformes/metabolism , Artemia
4.
Article in English | LILACS-Express | LILACS, VETINDEX | ID: biblio-1467277

ABSTRACT

Abstract Medicinal plants, such as E. pyramidale, are alternatives against infections and assist in the healing process of wounds. We evaluated the effects of Hydrogel of E. pyramidale on the healing of cutaneous wounds in animal models by morphological, morphometric and histological analyses, and elucidated major components. The ethanolic extract of E. pyramidale shoots was submitted to phytochemical analysis. For cicatrizing activity assay, Wistar rats were used, and the groups evaluated were hydrogel containing ethanolic extract of E. pyramidale at 2% compared to control groups. The treatment periods were 3, 7 and 21 days, and macroscopic and microscopic analyses were performed. Data were submitted to Analysis of Variance (p 0.05). Phytochemistry and quantification evidenced that flavonoids are main constituents and that they were evidenced by thin layer chromatography (TLC), high efficiency (HPLC) and infrared (IR) methods. In TLC, using flavonoid patterns, we observed pinocembrin. The hydrogel containing ethanolic extract of E. pyramidale at 2% was effective in wound regression. E. pyramidale can be used for the treatment of second intention wounds, and the effective healing may be due to a high flavonoid content.


Resumo As plantas medicinais, como E. pyramidale, são uma alternativa para combater infecções e ajudar o processo de cicatrização de feridas. Avaliar os efeitos do hidrogel de E. pyramidale na cicatrização de feridas cutâneas em modelos animais através de análises morfológicas, morfométricas e histológicas, bem como para elucidar os principais componentes. O extrato etanólico das partes aéreas de E. pyramidale foi submetido a análise fitoquímica. Para o ensaio de atividade cicatrizante, foram utilizados ratos Wistar e os grupos avaliados foram o hidrogel com extrato etanólico de E. pyramidale 2% em comparação com os grupos controle. Os períodos de tratamento foram 3, 7 e 21 dias e as análises macroscópicas e microscópicas foram realizadas e os dados submetidos à Análise de Variância (p 0,05). A fitoquímica e a quantificação indicaram que os flavonóides são os constituintes principais e que mesmos foram evidenciados na cromatografia de camada delgada (CCD), de alta eficiência (HPLC) e por infravermelho (IV). Em CCD, empregando padrões de flavonoides, foi observado a pinocembrina. O hidrogel com extrato etanólico de E. pyramidale 2% foi eficaz na regressão da ferida. E. pyramidale pode ser usado no tratamento de feridas de segunda intenção e a cura efetiva pode ser devido ao alto teor de flavonoides.

5.
Braz. j. morphol. sci ; 24(4): 208-210, Oct.-Dec.2004. ilus
Article in English | LILACS | ID: lil-658768

ABSTRACT

The extensor digitorum brevis manus is one of the rare anatomic variations which occur on the dorsum of the hand. Only some 295 articles were found worldwide in an extensive bibliographic review carried out in 2003. This muscle was dissected bilaterally on a male corpse at the Human Anatomy Laboratory of the Morphophysiological Department of the “Faculty of Medical Sciences of Minas Gerais”. It is an elongated, small muscle, originating at the carpal bones and at the extensor retinaculum and inserting into one of the tendons of the finger’s extensor muscle. Even though it does not present an essential function in the movement of the fingers nor the hand, it can lead to pain when hypertrophied, creating the need for clinical or even surgical treatment.


Subject(s)
Humans , Male , Female , Back , Back/physiology , Hand/anatomy & histology , Hand/pathology , Muscles/anatomy & histology , Cadaver , Diagnosis, Differential , Dissection
6.
Arq. bras. med. vet. zootec ; 50(3): 243-6, jun. 1998. tab
Article in Portuguese | LILACS | ID: lil-224103

ABSTRACT

Determinou-se a freqüência de bovinos soro-reagentes para Anaplasma marginale em rebanhos leiteiros das microrregiöes de Jequié, Itabuna e Vitória da Conquista, Estado da Bahia, pelas técnicas de imunoadsorçäo enzimática (ELISA), imunofluorescência indireta (IFI) e teste de conglutinaçäo rápida (TCR). Das 324 amostras de soro bovino analisadas, 96,9 por cento, 97,2 por cento e 91,0 por cento foram positivas para anticorpos contra A. marginale, respectivamente pelo ELISA, IFI e TCR. Todas as regiöes caracterizaram-se por estabilidade enzoótica para a anaplasmose. O desempenho dos três testes sorológicos foi bastante similar na detecçäo de anticorpos contra A. marginale


Subject(s)
Animals , Anaplasma , Cattle , Anaplasmataceae Infections/epidemiology , Anaplasmataceae Infections/immunology
7.
Arq. bras. med. vet. zootec ; 44(4): 263-70, ago. 1992. tab
Article in Portuguese | LILACS | ID: lil-240038

ABSTRACT

Em amostras de sangue colhidas de 60 fêmeas adultas, criadas no Estado de Säo Paulo, realizou-se o exame sorológico visando a detecçäo de anticorpos contra o vírus da Artrite-Encefalite Caprina (VAEC) e Mycoplasma mycoides. O índice clínico (IC) foi obtido pelo uso de uma fita métrica. Näo foram encontradas diferenças estatísticas significativas entre as médias dos IC encontrados em animais sorologicamente positivos e negativos. Considerando-se como 6,0 cm o valor crítico do IC, encontrou-se uma sensibilidade relativa de 57 por cento e uma especificidade relativa de 61 por cento no emprego do IC para o diagnóstico da infecçäo pelo VAEC e Mycoplasma mycoides. Os resultados encontrados mostram que o IC, isoladamente, näo é um critério satisfatório para o diagnóstico da infecçäo pelo VAEC


Subject(s)
Animals , Female , Arthritis-Encephalitis Virus, Caprine , Goat Diseases , Goats
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